Method of differentiating/inducing bone marrow interstitial...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) C12N 5/077 (2010.01) C12N 5/0793 (2010.01) C12N 5/0797 (2010.01) A61K 35/30 (2006.01) C12N 5/10 (2006.01) C12N 15/12 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2475288

It is intended to provide a method of differentiating/inducing bone marrow interstitial cells into nerve cells and skeleton muscle cells by transferring a Notch gene. Namely, a method of differentiating/inducing bone marrow interstitial cells into nerve cells or skeleton muscle cells in vitro which comprises transferring a Notch gene and/or a Notch signaling-associated gene into the above cells, wherein the differentiated/induced cells finally produced are obtained as the results of the cell division of the bone marrow interstitial cells having the Notch gene and/or the Notch signaling-associated gene transferred thereinto. It is also intended to provide a method of further differentiating/inducing the differentiated/induced cells as described above into dopaminergic neurons and acetylcholinergic neurons. Moreover, it is intended to provide a method of treating a nerurodegenerative or myodegenerative disease using the nerve precursor cells, nerve cells or skeleton muscle cells produced by the above methods.

L'invention concerne une méthode de différenciation/d'induction de cellules interstitielles de moelle osseuse en cellules nerveuses et en cellules musculaires du squelette par transfert du gène Notch. Notamment, ladite invention a pour objet une méthode de différenciation/d'induction de cellules interstitielles de moelle osseuse en cellules nerveuses ou en cellules musculaires du squelette in vitro, cette méthode consistant à transférer un gène Notch et/ou un gène lié à la signalisation Notch dans les cellules susmentionnées. Les cellules différenciées/induites finalement produites sont donc obtenues comme des résultats de la division cellulaire des cellules interstitielles de moelle osseuse possédant le gène Notch et/ou le gène lié à la signalisation Notch transféré. Cette invention a également trait à une méthode de différenciation/d'induction de cellules différenciées/induites telles que susmentionnées en neurones dopaminergiques et en neurones acétylcholinergiques. En outre, ladite invention concerne une méthode de traitement d'une maladie neurodégénérative ou myodégénérative au moyen de précurseurs nerveux, de cellules nerveuses ou de cellules musculaires du squelette produites par les méthodes susmentionnées.

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