G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/533 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2432538
The present invention relates to a method of disrupting a conjugate comprising a biotin compound and a biotin-binding compound, said method comprising the step of contacting said conjugate with an effective amount of a substantially aqueous solution under conditions such that the conjugate is disrupted, preferably reversibly disrupted, thereby forming a biotin compound and a biotin-binding compound. In particular, the invention relates to a method of reversibly releasing a biotinylated moiety from a streptavidin ( or avidin) coated support. Uses of the methods in techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also encompassed by the present invention.
L'invention concerne un procédé de rupture d'un conjugué comprenant un composé de biotine et un composé de fixation à biotine. Ce procédé consiste à mettre en contact ledit conjugué avec une quantité efficace de solution pratiquement aqueuse dans des conditions permettant d'effectuer la rupture de ce conjugué, de préférence, la rupture réversible, ce qui permet d'obtenir un composé de biotine et un composé de fixation à biotine. Elle concerne, en particulier, un procédé de libération réversible d'une fraction biotinylée depuis un support revêtu par streptavidine (ou avidine). Elle concerne également des mises en application de ces procédés dans des techniques de détection, d'identification, de détermination, de purification, de séparation et/ou d'isolation de protéines ciblées ou de molécules d'acide nucléique.
Fetherstonhaugh & Co.
Magnetic Biosolutions Sweden Ab
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