Method of distinguishing inflammatory pathogen causing acute...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/09 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2717244

The present invention provides a method of distinguishing a respiratory pathogen causing acute respiratory infection, wherein a pathogen to be distinguished is a pathogen colonizing the respiratory tract, the method comprising: a) measuring a gene copy number derived from a subject's cells, in DNA prepared from a sample containing the respiratory secretions of the subject; b) measuring a pathogen-derived gene copy number in the DNA; c) calculating the relative number of the pathogen-derived gene copy number to the gene copy number derived from the subject's cells; and d) distinguishing whether or not the pathogen is the respiratory pathogen, based on a distinguishment reference value and the relative number.

L'invention porte sur un procédé pour distinguer un pathogène inflammatoire provoquant une infection respiratoire aiguë, le pathogène à distinguer étant un pathogène résidant dans le tractus respiratoire. Ce procédé comporte : (a) une étape pour compter les copies de gènes provenant des cellules du sujet dans l'ADN préparé à partir d'un échantillon contenant une sécrétion provenant du tractus respiratoire du sujet ; (b) une étape pour compter les copies de gènes provenant du pathogène dans l'ADN ; (c) une étape pour calculer la valeur relative du comptage des copies de gènes provenant du pathogène au comptage des copies de gènes provenant des cellules du sujet ; et (d) une étape pour distinguer si le pathogène est un pathogène inflammatoire provoquant ou non la maladie sur la base de la norme de distinction et de la valeur relative.

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