Method of dna sequencing

Details Method of dna sequencing Method of dna sequencing

G - Physics

01

N

150/8.5

G01N 33/68 (2006.01) C12Q 1/68 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 1258611

ABSTRACT OF THE DISCLOSURE A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein a set of four chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing chemistry and permit the detection and characterization of the fragments as they are resolved by electrophoresis through a gel. A system for the analysis of DNA fragments comprising: a source of chromophore or fluorescent tagged DNA fragments, a zone for contacting an electrophoresis gel; means for intro- ducing said tagged DNA fragments to said zone; and photometric means for monitoring said tagged DNA fragments as they move through said gel.

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