Method of efficient extraction of protein from cells

Details Method of efficient extraction of protein from cells Method of efficient extraction of protein from cells

C - Chemistry, Metallurgy

07

K

C07K 1/14 (2006.01) C07K 1/34 (2006.01) C07K 14/005 (2006.01) C07K 14/025 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2705765

Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.

L'invention concerne des procédés de production d'un extrait protéique à partir de cellules, tels que des cellules ou des échantillons cellulaires contenant des protéines virales. De manière générale, les procédés peuvent impliquer : l'augmentation du pH des cellules jusqu'à un pH d'au moins environ pH 10,0 pour produire une composition intermédiaire, puis, en présence d'un détergent non ionique comme l'éther alkylique de polyoxyéthylène, la neutralisation du pH de la composition intermédiaire pour produire l'extrait protéique. Ces procédés peuvent être utilisés en combinaison avec des procédés de détection d'une ou plusieurs protéines cibles dans un échantillon, telles que des protéines virales. L'invention concerne également des systèmes, des nécessaires et des compositions pour mettre en uvre les procédés sujets.

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