C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/64 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2460388
-27- The object of the present invention is to solve the problems in excising a DNA region (or gene) with conventional restriction enzymes, or in amplification of the DNA region by PCR. The present invention provides a method for precisely, quickly and simply cloning a target ORF, for example the target ORF encoding a protein, particularly the target ORF encoding a long-chain ORF comprising bases as many as several thousand by in cDNA, and means such as various vectors used for the cloning method.
L'invention concerne la solution de problèmes survenant lors des procédés courants de coupure d'une région d'ADN (ou d'un gène) avec une enzyme de restriction ou d'amplification d'une région d'ADN par PCR. L'invention concerne en outre un procédé de clonage de manière précise, rapide et appropriée d'une ORF cible, par exemple une ORF codant pour une protéine, en particulier, une ORF à chaîne longue ayant plusieurs milliers de bps dans l'ADNc par utilisation d'une réaction de recombinaison homologue et de moyens tels que divers vecteurs servant dans ce procédé.
Nagase Takahiro
Nakajima Daisuke
Ohara Osamu
Kazusa Dna Research Institute Foundation
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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