Method of electrochemical detection of somatic cell mutations

Details Method of electrochemical detection of somatic cell mutations Method of electrochemical detection of somatic cell mutations

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2524265

The present invention relates to the detection of somatic cell mutations, particularly as part of a method to screen for cancer or precancer. The disclosure includes techniques for extracting and isolating oligonucleotides from a patient and conducting hybridization assays. Preferred embodiments include a combination of the following steps: extracting a biological sample from a patient, purifying a nucleic acid from a biological sample, amplifying a nucleic acid, isolating a nucleic acid in single stranded form, cyclizing a nucleic acid, elongating a nucleic acid, controlling hybridization stringency, amplifying a nucleic acid on a chip, and detecting hybridization.

La présente invention concerne la détection de mutations de cellules somatiques, notamment sous forme d'étape d'un procédé pour identifier un cancer ou un précancer. La présente invention concerne des techniques pour extraire et isoler des oligonucléotides d'un patient et pour effectuer des essais d'hybridation. Des modes de réalisation préférés concernent une combinaison des étapes suivantes : prélever un échantillon biologique d'un patient, purifier un acide nucléique d'un échantillon biologique, amplifier un acide nucléique, isoler un acide nucléique sous une forme à simple brin, cycliser un acide nucléique, allonger un acide nucléique, commander une rigueur d'hybridation, amplifier un acide nucléique sur une puce et détecter une hybridation.

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