Method of elevating photosynthesis speed of plant by...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 1/00 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2463326

A method of transforming a C4 plant; and a method of overexpressing a foreign gene in a C4 plant. More specifically, a method of constructing a C4 plant showing an excellent photosynthesis ability even at a low temperature by overexpressing an enzyme constituting the photosynthesis pathway of the C4 plant. A C4 plant is transformed by using an expression cassette containing a promoter, a C4 plant genomic gene encoding an enzyme constituting the photosynthesis pathway under the regulation by the promoter and a terminator. It is preferable that the C4 plant genomic gene encoding an enzyme constituting the photosynthesis pathway is a C4 plant genome-origin PPDK gene or its variant. The above method is particularly useful in elevating the productivity of a C4 plant carrying PPDK (among all, elevating the productivity of corn under cold conditions).

L'invention concerne un procédé de transformation de plante C4, et un procédé de surexpression de gène étranger dans une plante C4. Plus spécifiquement, l'invention concerne un procédé relatif à l'élaboration d'une plante C4 à excellente capacité de photosynthèse, même à basse température, qui consiste en une surexpression d'enzyme constituant le trajet de photosynthèse de ladite plante C4. La transformation de cette plante repose sur l'utilisation d'une cassette d'expression renfermant un promoteur, un gène génomique de plante C4 codant une enzyme qui constitue le trajet de photosynthèse sous la régulation du promoteur, et un terminateur. De préférence, le gène génomique de la plante C4 codant une enzyme qui constitue le trajet de photosynthèse est un gène PPDK originaire du génome de la plante C4 ou son variant. Le procédé considéré est particulièrement utile pour améliorer la productivité d'une plante C4 porteuse de PPDK (entre autres, pour améliorer la productivité du maïs dans des conditions froides).

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