C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/74 (2006.01) A23C 9/127 (2006.01) C12N 1/21 (2006.01) C12N 7/04 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01)
Patent
CA 2187624
-45- The present invention provides a method of identifying a bacterial DNA sequence capable of recombining with DNA of a predetermined bacteriophage the method comprises the steps of: (a) introducing a bacteriophage defense mechanism into a bacterium; (b) culturing the bacterium in the presence of a first lytic bacteriophage to produce a bacterial culture, the first lytic bacteriophage being sensitive to the bacteriophage defense mechanism; (c) isolating from the bacterial culture a second lytic bacteriophage which is resistant to the bacteriophage defense mechanism; (d) identifying DNA sequences present in the second lytic bacteriophage which are not present in the first lytic bacteriophage; and then (e) identifying a bacterial DNA sequence which is homologous to a DNA sequence of the second lytic bacteriophage identified in step (d); wherein the identified bacterial DNA sequence is capable of recombination with DNA of the first lytic bacteriophage.
L'invention concerne un procédé d'identification et de rupture de séquences bactériennes d'ADN contribuant à l'évolution de nouveaux bactériophages lytiques. Elle concerne également des vecteurs et des bactéries recombinantes s'utilisant dans la production de cultures de départ de fermentation et de milieux de culture résistant à l'apparition de nouveaux phages, ainsi que des procédés de production desdits vecteurs et desdites bactéries recombinantes.
Klaenhammer Todd R.
Moineau Sylvain
North Carolina State University
Sim & Mcburney
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