Method of enhancing immunogenicity by covalent linkage of...

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A61K 39/00 (2006.01) A01N 63/00 (2006.01) A61K 39/38 (2006.01) A61K 39/385 (2006.01) A61K 45/00 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2471251

A method is provided to increase the immunogenicity of an antigen. This method involves the covalent coupling of the antigen to proteins or glycoproteins present on the surface of dendritic cells by a mild biochemical modification which minimizes the denaturation of the antigen and preserving cell viability. Dendritic cells with covalently linked antigen on their surface can be used for generating a specific response to the antigen. The present method can be used for both therapeutic and preventive purposes.

L'invention concerne une méthode permettant de renforcer l'immunogénicité d'un antigène. Cette méthode implique, d'une part, la liaison covalente de l'antigène à des protéines ou à des glycoprotéines présentes à la surface de cellules dendritiques au moyen d'une légère modification biochimique qui minimise la dénaturation de l'antigène et, d'autre part, la préservation de la viabilité cellulaire. Les cellules dendritiques comportant, sur leur surface, un antigène lié par covalence peuvent servir à générer une réponse spécifique à l'antigène. Cette méthode peut servir à des fins thérapeutiques ou prophylactiques.

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