Method of enhancing the biological activity of ligands

C - Chemistry – Metallurgy – 12 – N

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C12N 15/62 (2006.01) C07K 14/515 (2006.01) C07K 14/75 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2354846

Novel fusion polypeptide ligands that bind Eph family receptors or the Tie-2 receptor are identified, and methods for making the fusion polypeptide ligands in biologically active form are described. Nucleic acids encoding these novel fusion polypeptide ligands enable production of the fusion polypeptide ligands. The method of making the nucleic acids and the fusion polypeptide ligands is broadly applicable to the production of polypeptide ligands in general, resulting in improved affinity and/or increased activity of the ligand when compared to its native form.

L'invention concerne de nouveaux ligands polypeptidiques de fusion qui se lient aux récepteurs de la famille des Eph ou au récepteur Tie-2, ainsi que des techniques permettant d'obtenir ces ligands dans des formes biologiquement actives. Les acides nucléiques codant ces nouveaux ligands polypeptidiques de fusion permettent la production desdits ligands. La technique de production des acides nucléiques et des ligands polypeptidiques de fusion peut être largement utilisée dans la production de ligands polypeptidiques en général, ce qui a comme résultat une affinité améliorée et/ou une activité accrue du ligand par rapport à sa forme native.

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