Method of enhancing wound healing by stimulating fibroblast...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/00 (2006.01) A61K 38/10 (2006.01) A61K 38/16 (2006.01) A61K 38/17 (2006.01) C07K 7/08 (2006.01) C07K 14/435 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2188391

A method of treating a wound of a mammalian subject in need of such treatment, to promote healing thereof, comprising administering to the subject, e.g., to the wound locus, a composition comprising a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide, preferably an amphipathic peptide which is antimicrobially effective at such locus. A method is also disclosed of stimulating the accelerated growth of dermal tissue in a tissue culture containing same, comprising applying to the tissue culture a fibroblast and keratinocyte proliferatingly effective amount of an amphipathic peptide, by which the dermal tissue may be grown to produce skin for skin grafting purposes, utilizing a dermal tissue culture containing dermal tissue material of a skin graft recipient of such skin. Novel amphipathic peptides suitable for use in such methods are disclosed.

Un procédé qui permet de traiter une blessure chez un mammifère pour en promouvoir la guérison, consiste à administrer au sujet, sur le site de la blessure par exemple, une composition comprenant une quantité efficace d'un peptide amphipathique chargée de faire proliférer des fibroblastes et des kératinocytes, ce peptide présentant de préférence en un tel site une efficacité anti-microbienne. On décrit aussi un procédé, qui permet de stimuler la croissance accélérée d'un tissu dermique dans une culture de tissu le contenant, et qui consiste à apporter à cette culture une quantité efficace d'un peptide amphipathique chargée de faire proliférer des fibroblastes et des kératinocytes, ce qui permet de faire croître ce tissu dermique pour produire de la peau susceptible d'être greffée, en utilisant une culture de tissu dermique qui contient du tissu dermique d'un receveur de greffe appelé à recevoir cette peau. On décrit enfin de nouveaux peptides amphipathiques propres à être utilisés avec de tels procédés.

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