Method of enriching and/or separating prokaryotic dna by...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01)

Patent

CA 2558515

The invention relates to a method for separating and/or enriching prokaryotic DNA, comprising the following steps: a) contacting of at least one prokaryotic DNA that is in solution with a protein that bonds specifically to prokaryotic DNA, said protein being 25 %-35 % homologous with the wild-type CGPB protein, thus forming a protein-DNA complex; and b) separation of the complex. The invention also relates to a kit for carrying out said method.

La présente invention concerne un procédé pour séparer et/ou concentrer de l'ADN procaryote. Ce procédé consiste a) à mettre en contact au moins un ADN procaryote se trouvant en solution avec une protéine qui se lie de manière spécifique à un ADN procaryote, la protéine présentant une homologie de 25 % à 35 % à une protéine CGBP de type sauvage, ce qui conduit à la formation d'un complexe protéine-ADN, puis b) à séparer ce complexe. La présente invention concerne également un kit pour mettre en oeuvre ledit procédé.

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