Method of enumerating escherichia coli

C - Chemistry – Metallurgy – 12 – Q

Patent

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150/15.2, 150/3.

C12Q 1/04 (2006.01) C07H 21/00 (2006.01) C12Q 1/10 (2006.01) C12Q 1/34 (2006.01) G01N 21/78 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1317534

ABSTRACT OF THE DISCLOSURE The present invention provides a method which is specific to determining and is capable of enumerating Escherichia coli (E. coli) in a sample water or sewage specimen. The method comprises adding to a cultured specimen a chromogenic reagent which when subjected to in situ E. coli .beta. -glucuronidase activity produces clearly defined vivid colourization of any individual colonies derived from E. coli cells in the test specimen.

532191

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