C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12N 15/09 (2006.01) C12P 19/32 (2006.01)
Patent
CA 2595873
A method of producing 3~-phosphoadenosine-5~-phosphosulfate (PAPS) characterized in that, in a method comprising using adenosine 5~-triphosphate sulfurylase (ATPS) and adenosine 5~-phosphosulfate kinase (APSK) and phosphorylating ATP to thereby produce PAPS, use is made of, as a substitute for ATP, an adenosine 5~-triphosphate (ATP)-supplying/regenerating system comprising adenosine 5~-monophosphate (AMP), polyphosphoric acid, polyphosphoric acid-depending nucleoside 5~-diphosphate kinase (PNDK) and polyphosphoric acid:AMP phosphotransferase (PAP), or an adenosine 5~- triphosphate (ATP)-supplying/regenerating system comprising adenosine 5~- monophosphate (AMP), polyphosphoric acid, polyphosphoric acid-depending nucleoside 5~-diphosphate kinase (PNDK) and adenylate kinase (ADK).
La présente invention décrit une méthode de synthèse de la 3~-phosphoadénosine-5~-phosphosulfate (PAPS) caractérisée en ce que, dans un procédé impliquant l'emploi de l'adénosine 5~-triphosphate sulfurylase (ATPS) et de l'adénosine 5~-phosphosulfate kinase (APSK) et la phosphorylation de l~ATP pour obtenir PAPS, il est employé, en remplacement de l~ATP, un système d'alimentation en/régénération de l'adénosine 5~-triphosphate (ATP) comprenant de l'adénosine 5~-monophosphate (AMP), de l'acide polyphosphorique, un nucléoside dépendant de l'acide polyphosphorique, de la 5~-diphosphate kinase (PNDK) et de l~acide polyphosphorique:AMP phosphotransférase (PAP), ou un système d'alimentation en/régénération de l'adénosine 5~-triphosphate (ATP) comprenant de l'adénosine 5~-monophosphate (AMP), de l'acide polyphosphorique, un nucléoside dépendant de l'acide polyphosphorique, de la 5~-diphosphate kinase (PNDK) et de l'adénylate kinase (ADK).
Ishige Kazuya
Noguchi Toshitada
Gowling Lafleur Henderson Llp
Yamasa Corporation
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