Method of evaluation of antioxidation level through...

Details Method of evaluation of antioxidation level through... Method of evaluation of antioxidation level through...

G - Physics

01

R

G01R 33/60 (2006.01) G01N 24/10 (2006.01) G01N 33/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2452276

It is intended to provide a method of evaluating antioxidation ability by measuring redox balance in vivo wherein the redox balance is represented in accordance with the following formula: redox balance = (antioxidation index after administration/oxidation index after administration)/(antioxidation index before administration/ oxidation index before administration). Each antioxidation index is obtained by quantitatively measuring the attenuation rate of signal strength of DMPO-OOL radical, which arises by supplementing butylhydroperoxide radical with 5,5-dimethyl-1-pyrrloine-N-oxide (DMPO), caused by the addition of serum with the use of a magnetic resonance spinning device and then indexizing the thus obtained antioxidation ability in accordance with 10-rank distribution (1 to 10). ON the other hand, each oxidation index is obtained by quantifying urinary 8-hydroxydeoxyguanosine (8- OHdG) and then indexizing the thus obtained oxidation ability in accordance with 10-rank distribution (1 to 10).

L'invention concerne l'obtention d'un procédé d'évaluation d'une capacité d'antioxydation par mesure d'un équilibre rédox in vivo, l'équilibre rédox représenté selon la formule suivante: équilibre rédox = (indice d'antioxydation après administration/indice d'oxydation après administration)/(indice d'antioxydation/indice d'oxydation avant administration). Chaque indice d'antioxydation est obtenu par mesure quantitative du taux d'atténuation de l'intensité du signal du radical DMPO-OOL, lequel s'élève par ajout au radical butylhydroperoxyde d'un 5,5-diméthyl-1-pyrrloine-N-oxyde (DMPO) provoqué par l'addition de sérum à l'aide d'un dispositif de giration à résonance magnétique et ensuite indexation de la capacité d'antioxydation ainsi obtenue selon une répartition à 10 niveaux (1 à 10). D'autre part, chaque indice d'oxydation est obtenu par quantification de la 8-hydroxydésoxyguanosine urinaire (8-OhdG) et ensuite indexation de la capacité d'oxydation ainsi obtenue selon la répartition à 10 niveaux (1 à 10).

Affiliated with

Also associated with

Rating

Method of evaluation of antioxidation level through... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of evaluation of antioxidation level through..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of evaluation of antioxidation level through... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1798123