Method of evaluation of the relative risk of developing...

G - Physics – 01 – N

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G01N 33/52 (2006.01)

Patent

CA 2606533

The present invention relates to a method for determining the amount of circulating CD36 protein or a fraction thereof which is present in cell-free plasma, preferably in a high molecular weight plasma fraction, such as a lipoprotein fraction selected from Low Density Lipoprotein, Intermediate Density Lipoprotein, and Very Low Density Lipoprotein using an immunological method which comprises the steps of (i) providing a plasma sample to be investigated, (ii) providing an anti-CD36 antibody, (iii) exposing the sample to be investigated to the antibody, and (iv) detecting and quantifying the amount of CD36 which binds to the antibody.

L'invention concerne un procédé pour déterminer la quantité de la protéine CD36 circulante ou une de ses fractions qui est présente dans un plasma sans cellules, de préférence une fraction de plasma à poids moléculaire élevé, telle qu'une fraction de lipoprotéines sélectionnée parmi une lipoprotéine à faible densité, une lipoprotéine à densité intermédiaire, et une lipoprotéine à très faible densité au moyen d'un procédé immunologique, comprenant les étapes suivantes : (i) production d'un échantillon plasma à examiner, (ii) production d'un anticorps anti-CD36, (iii) exposition de l'échantillon à examiner à l'anticorps, et (iv) détection et quantification de la quantité de CD36 qui est liée à l'anticorps.

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