Method of examining cancer cells and reagent therefor

Details Method of examining cancer cells and reagent therefor Method of examining cancer cells and reagent therefor

G - Physics

01

N

G01N 33/547 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01)

Patent

CA 2517580

A method whereby cancer cells can be conveniently and efficiently examined without resort to any expensive apparatus and a reagent therefor. In this cancer cell examination method, cancer cells expressing SF-25 antigen on the cell surface, which have been isolated from a living body, are bonded to magnetic beads by taking advantage of the antigen-antibody reaction between the cancer cells and anti-SF-25 antibody or an antigen-binding fragment thereof. Next, the magnetic beads are collected by magnetic attraction and then the cancer cells bonded on the magnetic beads are examined.

L'invention concerne des procédés dans lequel des cellules cancéreuses peuvent être examinées de manière pratique et efficace sans qu'il soit nécessaire d'avoir recours à un appareil onéreux. L'invention concerne également un réactif associé. Dans ce procédé d'examination de cellules cancéreuses, les cellules cancéreuses exprimant l'antigène SF-25 sur la surface cellulaire, qui ont été isolées à partir d'un corps vivant, sont collées à des billes magnétiques en profitant de la réaction antigène/anticorps entre les cellules cancéreuses et l'antigène anti-SF-25 ou un fragment de liaison antigène associé. Puis les billes magnétiques sont recueillies par attraction magnétique, et ensuite les cellules cancéreuses collées sur les billes magnétiques sont examinées.

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