Method of excising and propagating genomic dna

Details Method of excising and propagating genomic dna Method of excising and propagating genomic dna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/47 (2006.01) C12N 15/08 (2006.01)

Patent

CA 2066632

2066632 9105042 PCTABS00004 A process for isolating, cloning and maintaining large genomic fragments of chromosomal DNA. This process includes the steps of introducing a marker into the chromosomal DNA to be cloned, inserting double-strand breaks into the chromosome followed immediately by combining the cells containing the chromosome with the fragmented marker to another cell line capable of harboring extrachromosomal DNA elements, and subsequently isolating genomic fragments by separating the extrachromosomal DNA from the chromosomal DNA of the other cell line.

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