C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/071 (2010.01) A01K 67/027 (2006.01) C12N 15/09 (2006.01) C12N 15/85 (2006.01) C12N 15/861 (2006.01)
Patent
CA 2689591
Described herein is a method of expanding human hepatocytes in vivo using an immunodeficient mouse which is further deficient in fumarylacetoacetate hydrolase (Fah). The method comprises transplanting human hepatocytes into the immunodeficient and Fah-deficient mice, allowing the hepatocytes to expand and collecting the expanded human hepatocytes. The method also allows serial transplantation of the human hepatocytes into secondary, tertiary, quaternary or additional mice. Also provided are mutant mice comprising homozygous deletions or point mutations in the Fah, Rag2 and 112rg genes.
La présente invention a pour objet un procédé d'expansion in vivo d'hépatocytes humains à l'aide d'une souris immunodéficiente qui est en outre déficiente en fumaryl-acétoacétate hydrolase (Fah). Le procédé comprend la transplantation d'hépatocytes humains dans les souris immunodéficientes et déficientes en Fah, permettant l'expansion des hépatocytes et le recueil des hépatocytes humains qui ont subi l'expansion. Le procédé permet également une transplantation en série des hépatocytes humains dans des souris secondaires, tertiaires, quaternaires ou supplémentaires. L'invention concerne également des souris mutantes comprenant des délétions homozygotes ou des mutations ponctuelles dans les gènes Fah, Rag2 et 112rg.
Al-Dhalimy Muhsen
Azuma Hisaya
Grompe Markus
Kay Mark A.
Oregon Health & Science University
Smart & Biggar
The Board Of Trustees Of The Leland Stanford Junior University
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