Method of expressing gene in transgenic birds using...

A - Human Necessities – 01 – K

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A01K 67/027 (2006.01) C07K 16/00 (2006.01) C07K 16/02 (2006.01) C07K 16/28 (2006.01) C12N 15/13 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2492927

It is intended to provide G0 transgenic chimeric birds which have an antibody gene having been transferred thereinto by using a retrovirus vector with a lack of replicability and produce the antibody by expressing the transferred gene; a process for producing an antibody by collecting the antibody expressed by the constructed G0 transgenic chimeric birds; and a method of constructing G0 transgenic birds by incubating fertilized bird eggs and injecting a retrovirus vector into these fertilized eggs excluding immediately after the initiation of the incubation. Namely, G0 transgenic chimeric birds having an antibody gene transferred thereinto by using a retrovirus vector with a lack of replicability and producing the antibody by expressing the thus transferred gene.

La présente invention concerne des oiseaux chimériques transgéniques GO qui possèdent un gène d'anticorps qui a été transféré en eux au moyen d'un vecteur rétroviral sans réplicabilité et ce vecteur produit cet anticorps en exprimant ce gène transféré. Cette invention concerne aussi un processus de production d'un anticorps par la collecte de l'anticorps exprimé par ces oiseaux chimériques transgéniques GO construits et une technique de construction d'oiseaux transgéniques GO par incubation d'oeufs d'oiseau fertilisés et par l'injection d'un vecteur rétroviral dans ces oeufs fertilisés en excluant la période suivant immédiatement le début de l'incubation. Ces oiseaux chimériques transgéniques GO possèdent un gène d'anticorps transféré en eux au moyen d'un vecteur rétroviral sans réplicabilité et ces oiseaux produisent l'anticorps en exprimant ce gène ainsi transféré.

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