Method of expressing protein having unnatural amino acid...

Details Method of expressing protein having unnatural amino acid... Method of expressing protein having unnatural amino acid...

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2503749

A method of expressing a protein having an unnatural amino acid integrated thereinto characterized by comprising expressing (A) a mutant tyrosyl tRNA synthase which is a mutant of Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for a unnatural tyrosine derivative compared with the specificity for tyrosine, (B) a suppressor tRNA originating in an eubacterium belonging to the genus Bacillus, Mycoplasma or Staphylococcus which is capable of binding to the above tyrosine derivative in the presence of the above mutant TyrRS, and (C) a desired protein gene having a nonsense mutation at a desired site in animal cells to thereby incorporate the above tyrosine derivative into the nonsense mutation site in the above protein.

L'invention concerne un procédé permettant l'expression d'une protéine contenant un acide aminé non naturel. Ce procédé est caractérisé par l'expression (A) d'une tyrosyl-ARNt synthétase mutante qui est une forme mutante d'une tyrosyl-ARNt synthétase de Escherichia coli et présente une spécificité élevée vis-à-vis d'un dérivé de tyrosine non naturel par comparaison à sa spécificité vis-à-vis de la tyrosine, (B) d'un ARNt suppresseur provenant d'une eubactérie des genres bacille, mycoplasme ou staphylocoque, qui est capable de se lier avec ces dérivés de tyrosine, en présence du mutant TyRS décrit, et (C) du gène d'une protéine souhaitée comprenant une mutation non-sens dans un site donné, dans des cellules animales, afin d'incorporer les dérivé de tyrosine décrit dans le site de mutation non-sens de cette protéine.

Affiliated with

Also associated with

Rating

Method of expressing protein having unnatural amino acid... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of expressing protein having unnatural amino acid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of expressing protein having unnatural amino acid... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1363988