Method of expression of foreign gene in plant induced by...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2679972

There is provided an expression method of a target foreign gene in a plant induced by a chemical substance, which comprises a step of activating with copper ions a transcription factor encoded by a foreign gene different from the target foreign gene to induce activation of transcription of the target foreign gene by a region having a promoter function contained in the target foreign gene, wherein a nucleotide sequence encoding said transcription factor is contained in a gene construct which is constructed so as to comprise a nucleotide sequence of a 5'-untranslated region of an arbitrary foreign gene downstream from the region having a promoter function contained in the target foreign gene.

La présente invention concerne un procédé d'expression de gène étranger cible dans une plante induite par une substance chimique, comprenant une étape d'activation avec des ions de cuivre d'un facteur de transcription codé par un gène étranger différent du gène étranger cible pour induire l'activation de transcription du gène étranger cible par une région ayant une fonction promotrice contenue dans le gène étranger cible, la séquence nucléotidique codant pour ledit facteur de transcription étant contenue dans une construction génétique qui est construite de manière à inclure une séquence nucléotidique d'une région 5' non traduite d'un gène étranger arbitraire en aval de la région ayant une fonction promotrice contenue dans le gène étranger cible.

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