Method of fixing biomolecule to carrier

Details Method of fixing biomolecule to carrier Method of fixing biomolecule to carrier

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07K 1/04 (2006.01) G01N 33/53 (2006.01) G01N 33/545 (2006.01) C07B 61/00 (2006.01)

Patent

CA 2474091

A nucleic acid is fixed to a carrier made of a synthetic resin (for example, polycarbonate, polymethyl methacrylate, acrylonitrile-butadiene-styrene copolymer, polyethylene, polyethylene terephthalate, polyphenol, polystyrene, polyacrylonitrile, polyvinyl chloride or aramid) by spotting a solution of the nucleic acid on the carrier, drying the solution and then irradiating the carrier with UV rays containing a component of a wavelength of 280 nm preferably in a dose of 100 mJ/cm2 or more.

Selon l'invention, un acide nucléique est fixé sur un support constitué de résine synthétique (du polycarbonate, du polyméthyle méthacrylate, un copolymère acrylonitrile-butadiène-styrène, du polyéthylène, du polyéthylène téréphthalate, du polyphénol, du polystyrène, du polyacrylonitrile, du chlorure de polyvinyle ou de l'aramide). Le procédé selon l'invention consiste à déposer sur ledit support une solution formée à partir dudit acide nucléique; à sécher ladite solution; puis à irradier le support au moyen de rayons UV présentant une composante de longueur d'onde de 280 nm, de préférence à une dose supérieure ou égale à 100 mJ/cm?2¿.

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