C - Chemistry – Metallurgy – 07 – C
Patent
C - Chemistry, Metallurgy
07
C
260/472.15, 150/
C07C 69/76 (2006.01) C07F 9/04 (2006.01) C12Q 1/04 (2006.01) C12Q 1/34 (2006.01) C12Q 1/61 (2006.01)
Patent
CA 1154453
ABSTRACT OF THE DISCLOSURE: The object of the present invention is a new method for fluorometrically assaying the activity of fat-degrading enzymes, such as lipases and phospholipases in samples containing said enzyme, such as in serum. According to the method the enzyme containing sample is reacted with a subs- trate containing acyl- or alkylglycerols or -phosphoglycerols having at least one fluorescent group, such as a pyrene group. The compounds may in addition contain fluorescence quenching groups. The enzyme hydrolyzes the substrate thus giving rise to changes in the fluorescence intensity during the enzyme reaction and the changes in the intensity are measured at a specific emission wave-length of the fluorescent group employed. The rate of change of the invensity is proportional to the enzyme activity in the sample. The invention concerns also the new compounds containing fluorescent groups to be used in the method.
374931
Kinnunen Paavo K. J.
Schroder Tom M.
Virtanen Jorma A.
Ksv-Chemicals Oy
Robic Robic & Associes/associates
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