A - Human Necessities – 01 – N
Patent
A - Human Necessities
01
N
167/322
A01N 1/02 (2006.01) F25D 3/00 (2006.01) F25D 3/10 (2006.01)
Patent
CA 1197467
TITLE OF THE INVENTION: METHOD OF FREEZING FERTILIZED OVA, SPERMATOZOA OR THE LIKE AND APPARATUS THEREFOR ABSTRACT OF THE DISCLOSURE A method of freezing fertilized ova, spermatozoa or the like which has the steps of containing fertilized ova, spermatozoa or the like in a buffer solution at an irregular position in a tube, and cooling the same so that a buffer solution contained in an area containing no fertilized ova, spermatozoa or the like becomes lower at temperature than the buffer solution containing the fertilized ova, spermatozoa or the like with desired refrigerant to freeze the second buffer solution to produce crystalline nuclei, and then cooling the nuclei so that the nuclei grow to the first buffer solution. And, an apparatus for freezing fertilized ova, spermatozoa or the like which can carry out the above method. Thus, the survival rate of the fertilized ova, spermatozoa or the like can be raised, and the fertilized ova, spermatozoa or the like can be readily frozen under an automatic control by simple cooling means. BACKGROUND OF THE INVENTION This invention relates to a novel method of freezing fertilized ova, spermatozoa or the like and an apparatus for freezing the same adapted for carrying out the same method. An artificial fertilization has been recently carried frequently out to contrive the improvements of breed of domestic animal and of growth of the domestic animal. In this case, fertilized ova and spermatozoa have been
409052
Kuraoka Yasuo
Sakao Nobuo
Hoxan Corporation
Ridout & Maybee Llp
LandOfFree
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