Method of gene mapping

Details Method of gene mapping Method of gene mapping

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01)

Patent

CA 1322942

TITLE METHOD OF GENE MAPPING ABSTRACT The method of described characterizes each DNA segment to be mapped by cleaving it to produce DNA fragments which are then end labeled with a reporter(s) specific to the end nucleotides of each fragment. The labeled fragments are again cleaved to produce short fragments which are separated according to size. The short fragments are analyzed as to reporter identity and size which is indicative of the character of each fragment. By derivatizing the cleaved ends of the primary cleaved fragments, the labeling may be delayed until the second cleavage. Prior to labeling the derivatized fragments, all underivatized fragments are removed, the derivatized fragments being immobilized.

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