Method of gene screening with yeast that inducibly expresses...

Details Method of gene screening with yeast that inducibly expresses... Method of gene screening with yeast that inducibly expresses...

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 1/19 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2586182

An expression of an enzyme for synthesis routes for ergosterols, such as ERG6 or ERG3, is regulated with a promoter capable of induction. The enzyme in the expressed state is subjected to transformation. This enzyme in an expression- repressed state is used to conduct the screening or assay of a drug-resistant colony.

L'expression d'une enzyme intervenant dans les voies de synthèse des ergostérols, tels que ERG6 ou ERG3, est régulée à l'aide d'un promoteur capable d'induction. Dans son état exprimé, l'enzyme est soumise à transformation. Dans un état d'expression réprimée, ladite enzyme est employée afin de mener le criblage ou le dosage d'une colonie résistante aux médicaments.

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