Method of growing sperm stem cells in vitro, sperm stem...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/076 (2010.01) A01K 67/02 (2006.01) A01K 67/027 (2006.01) A61B 17/43 (2006.01) A61K 35/52 (2006.01) A61K 38/18 (2006.01) A61K 48/00 (2006.01) A61P 15/08 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2522050

It is intended to provide a method of growing sperm stem cells of a mammal or the like in vitro characterized in that glial cell line-derived neutrophic factor (GDNF) or its equivalent and leukemia inhibitory factor (LIF) are added to a medium (a culture liquor) for culturing sperm stem cells.

L'invention concerne une méthode de culture de cellules souches spermatiques d'un mammifère ou analogue in vitro, se caractérisant par le fait que le facteur de croissance neurotrophique issu des cellules gliales (GDNF) ou son équivalent et le facteur inhibiteur de la leucémie (LIF) sont ajoutés à un milieu (liqueur de culture) pour la culture de cellules souches spermatiques.

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