Method of identification of functional rna motifs in genomic...

G - Physics – 06 – F

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G06F 19/00 (2006.01) C12Q 1/68 (2006.01) G06F 7/20 (2006.01) G06F 17/30 (2006.01)

Patent

CA 2230739

The present invention relates to sequence data bases and more particularly, to the use of the information in these data bases to identify RNA motifs therein. More particularly, the invention relates to the use of a search engine which compares both primary and secondary structure of RNAs to identify functional RNA motifs in sequence data bases and to methods of identification of functionally relevant RNA motifs in sequence data bases using such search engines. The present invention further relates to the identification of specific RNA structural motifs in the sequence data bases such as the tat-binding element (TBE), the Rev-binding element (RBE), the hammerhead, a UV-loop motif, a neomycin binding motif and a paromomycin binding motif. In addition, the present invention relates to the validation of the approach of the present invention for indentifying functionally relevant RNA motifs by showing that a previously undiscovered RNA catalytic motif found in the Schistosome genome (DNA) sequence has catalytic activity.

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