Method of identifying and quantifying specific fungi and...

Details Method of identifying and quantifying specific fungi and... Method of identifying and quantifying specific fungi and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2412993

Fungi and bacteria can be detected and quantified by using a nucleotide sequence taught here that are specific to the particular species or group of species of fungi or bacteria. Use of the sequences can be made with fluorescent labeled probes, such as in the TaqMan.TM. system which produces real time detection of polymerase chain reaction (PCR) products. Other methods of detection and quanitification based on these sequences include hybridization, convention PCR or other molecular techniques.

Les champignons et les bactéries peuvent être détectés et quantifiés par utilisation de séquences nucléotidiques de l'invention spécifiques aux espèces ou au groupe d'espèces particuliers de champignons ou de bactéries. L'utilisation de ces séquences peut se faire avec des sondes fluorescentes étiquetées, telles que dans le système TaqMan.TM. qui produit une détection en temps réel de produits par réaction en chaîne de la polymérase (PCR). D'autres procédés de détection et de quantification basés sur ces séquences impliquent l'hybridation, la réaction en chaîne de la polymérase de convention ou d'autres techniques moléculaires.

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