Method of immobilizing an analyte on a solid surface

G - Physics – 01 – N

Patent

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Details

G01N 33/543 (2006.01) B05D 3/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/542 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2406091

Disclosed is a method for immobilizing an analyte on a solid surface, characterized by the following steps: - a cyclodextrin molecule having at least two functional groups is bonded to a solid surface so that at least one other functional group of the cyclodextrin molecule can be covalently bonded to the analyte; covalent bonding of the analyte to the surface-bonded cyclodextrin molecule. Alternately, the analyte can be initially covalently bonded and the cyclodextrin molecule can be subsequently bonded to the solid surface.

La présente invention concerne un procédé permettant d'immobiliser un analyte sur une surface solide. Ce procédé est caractérisé en ce qu'il consiste à fixer une molécule de cyclodextrine ayant au moins deux groupes fonctionnels sur une surface solide, de façon qu'encore au moins un groupe fonctionnel de la molécule de cyclodextrine puisse être lié de manière covalente à un analyte, puis à lier de manière covalente l'analyte à la molécule de cyclodextrine fixée sur la surface. En variante, l'analyte peut d'abord être lié de manière covalente à la molécule de cyclodextrine, puis la molécule de cyclodextrine peut ensuite être reliée à la surface solide.

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