Method of immobilizing single-stranded dna on carrier at...

Details Method of immobilizing single-stranded dna on carrier at... Method of immobilizing single-stranded dna on carrier at...

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2055755

- 18 - Abstract of the Disclosure- A method of immobilizing a single-stranded DNA on a carrier at the terminal, which comprises binding a double-stranded DNA comprising the single-stranded DNA and its complementary strand to the carrier only at the terminal of the single-stranded DNA and subsequently detaching the complementary strand from the single-stranded DNA by means of denaturing treatment, provided that the single-stranded DNA contains one or more extra nucleotide molecule(s) at the terminal when compared with the complementary strand or the terminal nucleotide molecule of the single-stranded DNA has been chemically modified so that the single-stranded DNA can bind to the carrier via the extra nucleotide molecule(s) or the chemically-modified terminal nucleotide molecule.

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