Method of immunological assaying of human osteocalcin,...

Details Method of immunological assaying of human osteocalcin,... Method of immunological assaying of human osteocalcin,...

C - Chemistry, Metallurgy

07

K

C07K 16/00 (2006.01) C07K 1/14 (2006.01) C07K 14/51 (2006.01) C07K 14/78 (2006.01) C07K 16/18 (2006.01) G01N 33/545 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2027108

- 59 - ABSTRACT A method of immunologically assaying intact human osteocalcin in a human assay sample by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-termianl side of human osteo- calcinand an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. A reagent and a kit therefor. Fur- thermore, a method of immunologicaslly assaying the total amount of human intact osteocalcin and a reagent thereof in a human assay sample. A reagent and a kit therefor. A monclonal antibody and a polyclonal antibody used for the assay. A process for producing these antibodies, and utilization of these antibodies.

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