Method of immunomodulation by means of adoptive transfer of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) A61K 35/14 (2006.01)

Patent

CA 2190261

A method for the production of antigen-specific cytotoxic T-cells (CTLs) by incubating a T cell-containing cell preparation with said antigen and isolating said antigen-specific CTLs, characterized by i) activating proliferation of CTLs in the cell preparation by incubation with the antigen; ii) selectively transferring a vector into the proliferating CTLs, said vector containing a marker gene by means of which marked and non-marked cells can be separated; and iii) separating the transfected antigen-specific CTLs on the basis of said marker gene, is useful for immunomodulation.

Procédé de production de lymphocytes T cytotoxiques spécifiques d'un antigène, par incubation, à l'aide de cet antigène, d'une préparation cellulaire renfermant des lymphocytes T, et par isolement desdits lymphocytes T cytotoxiques spécifiques de l'antigène. Le procédé est caractérisé par (i) l'activation de la prolifération des lymphocytes T cytotoxiques dans la préparation cellulaire par incubation à l'aide de l'antigène; (ii) le transfert sélectif d'un vecteur dans les lymphocytes T cytotoxiques en prolifération, ledit vecteur renfermant un gène marqueur permettant la séparation des cellules marquées et non marquées; et (iii) la séparation des lymphocytes T cytotoxiques spécifiques de l'antigène ainsi transfectés, en fonction dudit gène marqueur. Ledit procédé est utilisable dans l'immunomodulation.

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