Method of improving gene transfer efficiency into plant cells

Details Method of improving gene transfer efficiency into plant cells Method of improving gene transfer efficiency into plant cells

C - Chemistry, Metallurgy

12

N

C12N 15/84 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2386126

A method of improving the gene transfer efficiency into plant cells whereby a gene can be conveniently transferred without damaging tissues at a higher efficiency than in the conventional Agrobacterium gene transfer method. In this method, plant cells or plant tissues are heated and centrifuged to thereby improve the gene transfer efficiency into the plant cells mediated by a bacterium belonging to the genus Agrobacterium.

L'invention concerne un procédé permettant d'améliorer l'efficacité du transfert de gènes dans des cellules végétales, grâce auquel un gène peut être transférer de manière pratique sans endommager les tissus et de manière plus efficace que le procédé de transfert de gènes <i>Agrobacterium</i> classique. Selon le procédé décrit dans cette invention, les cellules végétales ou les tissus végétaux sont chauffés et centrifugés afin d'améliorer l'efficacité du transfert de gènes dans des cellules végétales à l'aide d'une bactérie appartenant au genre <i>Agrobacterium</i>.

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