Method of improving gene transfer efficiency into plant cells

Details Method of improving gene transfer efficiency into plant cells Method of improving gene transfer efficiency into plant cells

C - Chemistry, Metallurgy

12

N

C12N 15/84 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2386125

A method of improving the efficiency of transferring a gene into plant cells whereby gene transfer can be conveniently carried out without damaging the tissues at a higher efficiency than by the conventional Agrobacterium gene transfer method. In this method, plant cells or plant tissues are centrifuged to thereby improve the transfer of the gene into the plant cells mediated by a bacterium belonging to the genus Agrobacterium.

L'invention concerne un procédé permettant d'améliorer l'efficacité du transfert de gènes dans des cellules végétales, grâce auquel un gène peut être transférer de manière pratique sans endommager les tissus et de manière plus efficace que le procédé de transfert de gènes Agrobacterium classique. Selon le procédé décrit dans cette invention, les cellules végétales ou les tissus végétaux sont chauffés et centrifugés afin d'améliorer l'efficacité du transfert de gènes dans des cellules végétales à l'aide d'une bactérie appartenant au gène Agrobacterium.

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