C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/38 (2006.01) C07K 14/73 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 15/12 (2006.01) C12N 15/76 (2006.01)
Patent
CA 2090965
2090965 9206175 PCTABS00011 A method is provided for improving the yield of the heterologous proteins sCD4 and derivatives of sCD4, produced by recombinant Streptomyces lividans by the addition of casamino acids to the nutrient medium in which the bacteria are cultured. Also provided by this invention is a method for improving the half-life of heterologous proteins in a cell free culture supernatant by the addition of casamino acids to the supernatant. Complex media are also provided.
Arcuri Edward J.
Brawner Mary E.
Donovan Mary J.
Gerber Robert G.
Keller John A.
Gowling Lafleur Henderson Llp
Smithkline Beecham Corporation
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