Method of in situ detection of proteins using aptamers

Details Method of in situ detection of proteins using aptamers Method of in situ detection of proteins using aptamers

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07K 1/10 (2006.01) C12P 21/00 (2006.01) G01N 33/53 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2540472

The present invention is a method for the detection and localization of proteins in situ. The method comprises contacting a peptide tag labelled protein with a nucleic acid aptamer that recognizes the peptide tag. The nucleic acid aptamer is functionalized such that is detectable by a variety of methods. The method allows for the detection of one or more proteins and/or protein complexes in sub-cellular and sub-nuclear structures with high specificity and also allows for the purification of such identified proteins.

L'invention concerne un procédé de détection et de localisation de protéines in situ. Le procédé comporte l'étape consistant à mettre en contact une protéine munie d'une étiquette peptidique avec un aptamère d'acide nucléique qui reconnaît l'étiquette peptidique. Ledit aptamère est fonctionnalisé de manière à pouvoir être détecté à l'aide de diverses techniques. Ce procédé permet de détecter une ou plusieurs protéines et/ou complexes protéiniques dans des structures sous-cellulaires et sous-nucléaires avec une grande spécificité, et également de purifier les protéines identifiées.

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