Method of inactivating exonuclease activity

Details Method of inactivating exonuclease activity Method of inactivating exonuclease activity

C - Chemistry, Metallurgy

12

N

195/39, 195/76,

C12N 9/99 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1335962

A method of inactivating exonuclease activity in a DNA polymerase solution produced by recombinant DNA techniques, comprising: incubating said solution in a vessel containing oxygen, a reducing agent and a transition metal. Preferably the DNA polymerase is a T7-type DNA polymerase, especially T7 DNA polymerase.

616697

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