Method of inactivating ribonucleases at high temperature

Details Method of inactivating ribonucleases at high temperature Method of inactivating ribonucleases at high temperature

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2519907

Methods for protecting RNA from Rnase degradation and for inactivating Rnases in solution are disclosed. The invention includes methods for protecting RNA during storage, for performing quantitative PCR reactions, or for preparing cDNA. The method includes using a combination of an Rnase inhibitor in a solution containing or devoid of reducing agents and high heat to render Rnases inactive.

L'invention concerne une méthode permettant d'éviter que l'ARN ne subisse une dégradation sous l'effet de la Rnase et pour inactiver des Rnases en solution. L'invention comprend des méthodes de protection de l'ARN pendant stockage, pour effectuer des réactions quantitatives par réaction en chaîne de la polymérase ou pour préparer de l'ADNc. Ladite méthode comprend l'utilisation d'une combinaison d'un inhibiteur de Rnase dans une solution contenant des agents réducteurs ou en étant dépourvue et de chaleur élevée pour rendre les Rnases inactives.

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