Method of increasing expression of viral proteins

Details Method of increasing expression of viral proteins Method of increasing expression of viral proteins

C - Chemistry, Metallurgy

12

N

C12N 15/38 (2006.01) C07K 14/045 (2006.01) C07K 14/71 (2006.01) C12N 15/18 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2140531

Methods for the recombinant expression and secretion of viral proteins are disclosed. The methods involve the use of compatible escorts to shuttle the proteins to the cell surface. In this way, egress of recombinantly produced proteins out of the cell is facilitated, resulting in increased yields and easier purification of the desired protein.

Procédés d'expression et de sécrétion par recombinaison de protéines virales. Ces procédés consistent à utiliser des agents accompagnateurs compatibles afin d'entraîner les protéines en un mouvement de navette vers la surface cellulaire. De cette façon, la sortie de protéines produites par recombinaison de la cellule est facilitée, ce qui permet d'obtenir des rendements accrus et une purification plus aisée de la protéine requise.

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