C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) A23K 1/165 (2006.01) A23K 1/18 (2006.01) C12N 1/21 (2006.01) C12N 9/24 (2006.01) C12N 15/74 (2006.01)
Patent
CA 2136784
A method for increasing the catalytic activity of a glycanase having two or more independent catalytic domains by isolating the catalytic domains from the intact enzyme is described. If the catalytic domains in the intact enzyme are linked by a proline/hydroxyamino acid-rich linker sequence, the catalytic domains can be isolated and activated by cleavage within or adjacent to the linker sequence with a protease. The catalytic domains can also be isolated, whether or not they are linked by a hydroxyamino acid-rich linker sequence, by expressing the DNA sequences encoding each domain in separate transformed microbial hosts. An isolated and purified low-activity xylanase, denoted XynC, and which is obtained from Fibrobacter succinogenes is also described. XynC contains two independant active catalytic domains separated by a serine rich linker region. The enzyme has a mass of 63 kDa and an activity of 6 U/mg (µmol/min/mg) protein. The subcloned domains expressed separatedly exhibit 55 to 116 fold higher activity than the intact enzyme has a specific activity of approximately 6 U/mg protein and a mass of 63 kDa and consisting of two independently active catalytic domains linked by a hydroxyamino acid-rich linker sequence. XynC can be activated by treatment of the intact enzyme with a protease. The utilization of feed by domesticated animals and poultry can be improved by admixing the feed with XynC and a catalytic quantity of protease as the XynC is activated by proteases indigenous to the digestive tract of the animal.
Cheng Kuo Joan
Forsberg Cecil W.
Krell Peter John
Phillips John Paul
Department Of Agriculture And Agri-Food Canada (the)
Mckay-Carey & Company
LandOfFree
Method of increasing the catalytic activity of a glycanase... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of increasing the catalytic activity of a glycanase..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of increasing the catalytic activity of a glycanase... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1653371