Method of increasing the content of fatty acids in plant seeds

Details Method of increasing the content of fatty acids in plant seeds Method of increasing the content of fatty acids in plant seeds

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2375317

The invention relates to nucleic acid molecules that encode a protein with the activity of a .beta.-ketoacyl-ACP synthase IV (KASIV) from <i>Cuphea lanceolata</i>, to nucleic acid molecules that encode a protein with the activity of a .beta.-ketoacyl-ACP synthase II (KASII) from <i>Brassica napus</i>, and to nucleic acid molecules that encode a protein with the activity of a .beta.-ketoacyl-ACP synthase I (KASI) from <i>Cuphea lanceolata</i>. The invention further relates to a method of increasing the content of fatty acids, especially of short- and medium-chain fatty acids in triglycerides of plant seeds. The inventive method comprises expressing a protein with the activity of KASII or a protein with the activity of KASIV in transgenic plant seeds.

La présente invention concerne des molécules d'acide nucléique qui codent une protéine ayant l'activité d'une synthase IV .beta.-cétoacyl-PTA (KASIV) à partir de <i>Cuphea lanceolata</i>, des molécules d'acide nucléique qui codent une protéine ayant l'activité d'une synthase II .beta.-cétoacyl-PTA (KASII) à partir de <i>Brassica napus</i>, ainsi que des molécules d'acide nucléique qui codent une protéine ayant l'activité d'une synthase I .beta.-cétoacyl-PTA (KASI) à partir de <i>Cuphea lanceolata</i>. Cette invention concerne également un procédé pour augmenter la teneur en acides gras, notamment en acides gras à chaîne courte et moyenne, dans des triglycérides de graines de plantes. Le procédé selon l'invention comprend l'expression d'une protéine ayant l'activité d'une KASII et d'une protéine ayant l'activité d'une KASIV dans des graines de plantes transgéniques.

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