Method of inducing an immune response with a live venezuelan...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 39/12 (2006.01) A61K 39/193 (2006.01) C07K 14/155 (2006.01) C07K 14/165 (2006.01) C07K 14/18 (2006.01) C07K 14/285 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) C12N 15/40 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2193210

A method of protecting a subject against a disease comprises administering a recombinant Venezuelan Equine Encephalitis (VEE) virus to the subject in an effective immunogenic amount, with the VEE virus containing a heterologous DNA segment, and with the heterologous DNA segment comprising a promoter operable in the subject operatively associated with a DNA encoding an immunogenic protein or peptide effective for protecting the subject from the disease. Preferred promoters are VEE 26S subgenomic promoters, and preferred immunogens are viral immunogens. Novel attenuating mutations useful in carrying out the invention are also disclosed.

Le procédé de la présente invention permet la protection d'un sujet contre une affection. Il consiste à administrer au sujet une qqantité immunogéniquement efficace de virus de l'encéphalite équine du Venezuela contenant un segment d'ARN hétérologue, lequel comporte un promoteur fonctionnel dans le sujet et fonctionnellement associé à une séquence codant une protéine ou un peptide immunogène efficace pour protéger le sujet contre l'affection. Les promoteurs préférés sont les promoteurs sub-génomique 26S de l'encéphalite équine du Venezuela, les immunogènes préférés étant les immunogènes viraux. L'invention concerne également de nouvelles mutations atténuantes permettant la mise en oeuvre de l'invention.

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