Method of inducing gene expression in plant and plant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2376268

A method of inducing the expression of a gene under the regulation by an operator at an actinomycetes self-regulator provision site, which comprises imparting the characters of a repressor and an operator constituting a gene expression inducing system with the use of the actinomycetes self-regulator as an inducer to a plant by gene transfer, and then providing the actinomycetes self-regulator to the plant thus transformed. By using this method, a desired gene can be expressed at a desired point at a desired site of a plant, which makes it possible to produce a metabolite, which is even disadvantageous in the growth of the plant, in the plant. This method is also useful in controlling the fertility to thereby prevent transformed plants from scattering into the surroundings.

L'invention concerne un procédé destiné à induire l'expression d'un gène sous la régulation d'un opérateur à un site de service auto-régulateur d'actinomycètes. Ledit procédé consiste à conférer les caractères d'un répresseur et d'un opérateur formant un système induisant une expression génique avec l'utilisation de l'auto-régulateur d'actinomycètes comme inducteur vers une plante par transfert génique, puis à fournir l'auto-régulateur d'actinomycètes à la plante ainsi transformée. Par ce procédé, on peut exprimer un gène voulu à un point particulier d'un site voulu, rendant ainsi possible la production, dans la plante, d'un métabolite, ce qui défavorise même la croissance de la plante. Ce procédé permet également de réguler la fertilité et, par conséquent, d'empêcher que les plantes transformées se répandent aux alentours.

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