Method of inhibiting viral replication in eukaryotic cells...

A - Human Necessities – 61 – K

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A61K 31/4412 (2006.01) A61K 38/02 (2006.01) A61K 38/04 (2006.01) A61K 38/06 (2006.01) A61K 47/48 (2006.01) C12N 5/00 (2006.01) C12N 7/00 (2006.01) C12N 9/99 (2006.01)

Patent

CA 2223703

The present invention is directed to methods which inhibit post--translational hypusine formation in the intracellular protein eIF-5A, to suppress infections by virases that parasitize eIF-5A to promote their own replication. Inhibition of the post-translational formation of hypusine in infected host cells with hypusine inhibitors selectively suppresses the production of viral proteins and of infectious viral particles, and causes, particularly after hypusine inhibitor withdrawal, apoptosis in such virally-infected cells. This agent can be a compound of Formulae (I) or (II) and derivatives thereof as follows: R1, R2, R3, and R4 each individually represents a hydrogen, an alkyl, alkenyl, or alkoxy group containing 1 to about 8 carbon atoms, an aryl, aralkyl, or cycloalkyl group containing about 5 to 12 carbon atoms, or a carboalkoxy or carbamyl group containing up to 8 carbon atoms, or a peptide or peptidomimetic moiety containing 10 to about 30 carbon atoms.

L'invention se rapporte à des procédés qui permettent d'inhiber la formation post-translationnelle d'hipusine dans la protéine intra-cellulaire eIF-5A et d'annihiler les infections induites par des virus qui parasitent cette protéine afin de promouvoir leur propre réplication. L'inhibition de la formation post-translationnelle d'hypusine dans les cellules hôtes infectées, au moyen d'inhibiteur d'hypusine permet de supprimer de manière sélective la production des protéines virales et des particules virales infectieuses et, en particulier après le retrait des inhibiteurs d'hypusine, d'induire l'apoptose dans les cellules qui subissent l'infection virale. Cet agent peut être un composé représenté par les formules (I et II) et des dérivés de celui-ci. Dans lesdites formules, R1, R2, R3, et R4 représentent individuellement un groupe hydrogène, alkyle, alcényle ou alcoxy contenant 1 à 8 atomes de carbone environ, un groupe aryle, aralkyle ou cycloalkyle contenant environ 5 à 12 atomes de carbone, ou bien un groupe carboalcoxy ou carbamyle contenant jusqu'à 8 atomes de carbone, ou bien encore une fraction peptidique ou peptidomimétique contenant 10 à 30 atomes de carbone environ.

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