Method of introducing sirna into adipocytes

Details Method of introducing sirna into adipocytes Method of introducing sirna into adipocytes

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A61K 48/00 (2006.01) C12N 15/11 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2509494

In general, it has been difficult to introduce nucleic acid sequences into adipocytes. Methods are described for introducing nucleic acids such as siRNAs into adipocytes, including the introduction of sequences targeting Akt1, Akt2 and Myo 1c. Methods for identifying genes involved in glucose transport and for identifying insulin response modulators are described. Therapeutic methods are also provided.

Il s'avère, en général, difficile d'introduire des séquences d'acide nucléique dans des adipocytes. Selon cette invention, les procédés d'introduction des acides nucléiques tels que les siRNA dans des adipocytes comprennent l'introduction de séquences ciblant Akt1, Akt2 et Myo1c. L'invention porte également sur des procédés d'identification de gènes impliqués dans le transport du glucose et sur des procédés d'identification de modulateurs de la réponse insulinique. L'invention porte, en outre, sur des méthodes thérapeutiques.

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