Method of isolating an endothelial cell and method of donor...

Details Method of isolating an endothelial cell and method of donor... Method of isolating an endothelial cell and method of donor...

C - Chemistry, Metallurgy

12

Q

C12Q 1/24 (2006.01) C12N 5/071 (2010.01) C12Q 1/04 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2486119

Method of isolating an endothelial cell from a biological sample using detection reagent specific against an endothelial cell surface receptor (e.g. Tie-2). This isolated endothelial cell can be used in the detection of endothelial cell antibodies, this has been proven clinically important for successful organ transplantation. Disclosed are therefore also methods of isolating endothelial cell antibodies for donor-specific crossmatching.

La détection d'anticorps des cellules endothéliales s'est avérée importante au plan clinique pour la réussite d'une transplantation d'organe. L'invention porte sur des méthodes d'isolement des anticorps de cellules endothéliales en vue de l'essai de comptabilité croisée spécifique du donneur.

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