Method of isolating analytes from a sample

Details Method of isolating analytes from a sample Method of isolating analytes from a sample

G - Physics

01

N

G01N 33/543 (2006.01)

Patent

CA 2625499

The current invention is a capture-particle comprising: a) a molecular sieve portion; and b) an analyte binding portion; wherein the molecular sieve portion, analyte binding portion or both further comprise a cross-linked region having modified porosity. Capture particles wherein the molecular sieve portion, analyte binding portion or both comprise pore dimensions sufficient to exclude molecules larger than about 60 kDa. These particles are useful in purification and diagnostic methods. Kits comprising the capture particles are also described.

L'invention concerne une particule de capture comprenant: a) une partie de tamis moléculaire; et b) une partie de liaison à un analyte. La partie de tamis moléculaire, la partie de liaison à un analyte, ou les deux, comprennent également une zone réticulée présentant une porosité modifiée. Les particules de capture sont telles que la partie de tamis moléculaire, la partie de liaison à un analyte, ou les deux, présentent des dimensions de pore suffisantes pour exclure les molécules supérieures à 60 kDa environ. Ces particules sont utiles dans des méthodes de purification et de diagnostic. L'invention concerne également des trousses comprenant les particules de capture susmentionnées.

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